E-MTAB-2244 - Human splenic marginal zone precursors (MZP) differentiation into marginal zone B cells (MZB)through a Notch2 signalling pathway
Released on 26 March 2014, last updated on 3 June 2014
RNA microarray profiling analysis was performed on different ex-vivo human splenic B cell subsets (“Naïve”, marginal zone precursors or “MZP”, marginal zone B cells or” MZB”, “MTG+CD45+” cells) and on “p27” cells, corresponding to the fraction of MZP cells that differentiated into CD27-expressing cells after culture on OP9-hDLL1 cells cultured with OP9-hDLL1 cells that differentiated into CD27-expressing cells
transcription profiling by array, co-expression, ex vivo cell type comparison design and in vitro differentiation cell type comparison design, in vitro, in vivo
A splenic marginal zone B-cell precursor with NOTCH2-dependent differentiation properties in humans. Marc Descatoire1, Sandra Weller1, Sabine Irtan2, Jean Feuillard3, Sébastien Storck1, Anne Guiochon-Mantel4, Jérôme Bouligand4, Alain Morali5, Olivier Bernard6, Emmanuel Jacquemin6,7, Maria Iascone8, Christine Bole-Feysot9, Nicolas Cagnard10, Claude-Agnès Reynaud1,* and Jean-Claude Weill1,*.