E-MTAB-2241 - Transcription profiling by tiling array of wild-type or reb1-deleted budding yeast strains

Status
Last updated on 18 November 2014, released on 5 December 2014
Organism
Saccharomyces cerevisiae
Samples (1)
Array (1)
Protocols (4)
Description
Widely transcribed and compact genomes face the major challenge of coping with frequent overlapping or concurrent transcription events. Efficient and timely transcription termination is crucial to control pervasive transcription. In yeast, RNA polymerase II (RNAPII) termination mainly occurs via two pathways, one generating mRNAs and one dedicated to non-coding RNAs, and is triggered by signals that are recognized on the nascent RNA by a specific complex. We describe here a novel pathway of RNAPII transcription termination that is triggered by the binding to the DNA of the transcriptional activator Reb1p. We show that termination follows road-block induced pausing of RNAPII and requires ubiquitylation of RNAPII. The released RNAs are rapidly degraded, which defines a new class of cryptic unstable transcripts. We show that Reb1p-dependent termination can prevent transcriptional interference. This work reveals a novel role for Reb1p and a new paradigm for preserving the functional integrity of nucleosome free regions.
Experiment types
transcription profiling by tiling array, genetic modification design, growth condition design, reference design, replicate design
Contact
Citation
Road-block transcription termination by Reb1p enforces transcription boundaries.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-2241.idf.txt
Sample and data relationshipE-MTAB-2241.sdrf.txt
Raw data (2)E-MTAB-2241.raw.1.zip, E-MTAB-2241.raw.2.zip
Array designA-AFFY-116.adf.txt