E-MTAB-1929 - Transcription profiling by high throughput sequencing of Insm1+/− and Insm1−/− endocrine progenitor cell populations at embryonic day 15.5, Transcriptional regulation of endocrine cell differentiation by Insm1
Released on 1 July 2014, last updated on 1 July 2016
This experiment used RNA-Seq technology to explore gene expression in mouse Insm1^GFP/Pdx1^CFP HIGH [het/het] FACS sorted pancreatic cells (pre-beta cells) and Insm1^GFP/Pdx1^CFP LOW [het/het] cells (other endocrine progenitors) at E15.5 and E18.5. Comparison of Insm1 +/Pdx HIGH and Insm1 +/Pdx LOW cells revealed a set of differentially expressed genes that are required for beta cell specification.
RNA-seq of coding RNA, cell type comparison design, development or differentiation design
Insm1 promotes endocrine cell differentiation by modulating the expression of a network of genes that includes Neurog3 and Ripply3. Osipovich AB, Long Q, Manduchi E, Gangula R, Hipkens SB, Schneider J, Okubo T, Stoeckert CJ Jr, Takada S, Magnuson MA. , PMID:25053427