E-MTAB-189 - ChIP-Seq of human normal pancreatic islets with anti-histone antibodies to analyse histone modifications
Released on 18 February 2010, last updated on 3 June 2014
This experiment used ChIP-seq technology to create a genome-wide profile of histone marks in normal human pancreatic islets. In the current work we analyzed two histone marks associated with gene expression (H3K4me3, H3K4me1) and marks associated with gene repression(H3K27me3). Each mark was anayzed using samples obtained from four donors (n=4). Chromatin Immunoprecipitations (ChIPs) for histone marks were performed using specific anti-histone antibodies. Enrichment of each sample was calulated with respect to its individual input using qPCR. Samples were sequenced with Solexa and sequenced DNA from both Input (n=4) and ChIP (n = 4) samples were aligned to the NCBI Genome Build 36.1 Ð Hg18 to determine regions that were enriched for binding by modified histones.
ChIP-seq, chromatin immunoprecipitation, high throughput sequencing
Genome-wide analysis of histone modifications in human pancreatic islets. Bhandare R, Schug J, Le Lay J, Fox A, Smirnova O, Liu C, Naji A, Kaestner KH. Genome Res 20(4):428-433 (2010), PMID:20181961