E-MTAB-1371 - CLIP-Seq of H. sapiens HeLa cells to investigate transcriptome-wide mapping of hnRNP C and U2AF65

Status
Released on 31 January 2013, last updated on 3 May 2014
Organism
Homo sapiens
Samples (7)
Protocols (7)
Description
iCLIP experiment to assess the binding of the highly abundant nuclear RNA-binding protein hnRNP C and core splicing factor U2AF65 on a genomic scale. To investigate how both proteins compete for binding at a subset of sites, U2AF65 iCLIP experiments were performed from both HNRNPC knockdown and control HeLa cells.
Experiment types
CLIP-Seq, genetic modification, in vivo
Contact
Citations
Direct Competition between hnRNP C and U2AF65 Protects the Transcriptome from the Exonization of Alu Elements. Kathi Zarnack, Julian Konig, Mojca Tajnik, Inigo Martincorena, Sebastian Eustermann, Isabelle Stevant, Alejandro Reyes, Simon Anders, Nicholas M. Luscombe, Jernej Ule. Cell  (2012), PMID:23374342
Direct competition between hnRNP C and U2AF65 protects the transcriptome from the exonization of Alu elements. Zarnack K, König J, Tajnik M, Martincorena I, Eustermann S, Stévant I, Reyes A, Anders S, Luscombe NM, Ule J. :453-466 (2013), PMID:23374342
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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