E-MTAB-134 - Chromatin immunoprecipitation of mouse MIN6 pancreatic beta cells to identify Pdx1 targets
Released on 2 December 2009, last updated on 3 June 2014
The aim of this experiment was to use highthroughput chromatin immunoprecipitation followed by hybridization to promoter microarrays to obtain a comprehensive list of sites in the genome that are physically occupied by Pdx1. Chromatin was prepared from Min6 insulinoma cells and immunoprecipitated with Pdx1 or control antiserum. The precipitated chromatin was then purified, amplified and directly sequenced using Illumina technology.
transcription profiling by array, binding site identification, dye swap
David Groff <firstname.lastname@example.org>, Cynthia Khoo <email@example.com>, Doris Stoffers <firstname.lastname@example.org>, Jonathan Schug <email@example.com>, Juxiang Yang <firstname.lastname@example.org>, Kathryn Claiborn <email@example.com>, Mira Sachdeva <firstname.lastname@example.org>, Peter White <email@example.com>
Pdx1 (MODY4) regulates pancreatic beta cell susceptibility to ER stress. Sachdeva MM, Claiborn KC, Khoo C, Yang J, Groff DN, Mirmira RG, Stoffers DA. Proc Natl Acad Sci U S A. 106(45):19090-5 (2009)