E-MTAB-1147 - Transcription profiling by high throughput sequencing of HNRNPC knockdown and control HeLa cells
Released on 31 January 2013, last updated on 22 August 2016
We performed RNA-seq experiments on two replicate samples from each HNRNPC knockdown (KD1 and KD2) As well as from control HeLa cells. Library preparation was preformed according to mRNA Sequencing Sample Preparation Guide (Illumina, Part # 1004898 REV. D). Reagents were taken from the Illumina sample preparation kit (Illumina, CAT # RS-930-1001). Knockdown and control samples were sequenced together in one flowcell on one and two lanes, respectively.
RNA-seq of coding RNA, cellular modification, co-expression, in vitro
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A novel human-specific splice isoform alters the critical C-terminus of Survival Motor Neuron protein. Seo J, Singh NN, Ottesen EW, Lee BM, Singh RN. :30778 (2016), PMID:27481219