E-MTAB-1091 - RNA-seq of liver samples from C57BL/6J, CAST/EiJ mice as well as their initial and reciprocal F1 progeny to study the compensatory cis-trans regulation of liver gene expression
Released on 7 September 2012, last updated on 3 May 2014
Two inbred mouse strains, C57BL/6J and CAST/EiJ, were crossed to generate both initial and reciprocal F1 crosses. For each genetically distinct class of mice (F0 C57BL/6J, F0 CAST/EiJ, F1i - C57BL/6J x CAST/EiJ, F1r - CAST/EiJ x C57BL/6J, where the male parent is listed first), samples were collected from a single lobe of the liver from 6 male mice between the ages of 4 and 6 months. The 24 samples were then processed to generate strand-specific RNA-seq libraries, which were sequenced on the Illumina GAII platform using 72bp paired-end reads.
RNA-seq of coding RNA, imprinting, strain or line
Extensive compensatory cis-trans regulation in the evolution of mouse gene expression. Goncalves A, Leigh-Brown S, Thybert D, Stefflova K, Turro E, Flicek P, Brazma A, Odom DT, Marioni JC. Genome Res (2012), PMID:22919075