E-MTAB-10265 - Microarray analysis of Caenorhabditis elegans exposed to three hydrophilic genotoxicants in water

Status
Last updated on 22 April 2021, released on 23 April 2021
Organism
Caenorhabditis elegans
Samples (24)
Array (1)
Protocols (6)
Description
In this study, we exposed Caenorhabditis elegans wild types N2 to two concentrations of the model genotoxicants formaldehyde (HCHO), N-ethyl-N-nitrosourea (ENU), and methyl methanesulfonate (MMS).We performed a concentration-response test to determine the non-toxic concentration range for studying the transcriptional effects of compounds. In microarray experiments, we studied two concentrations (1 mM and 5 mM) for each compound and a control of M9 medium. Age synchronized worms at developmental L4 larval stage were exposed to treatment for four hours. After flash freezing the samples, RNA was isolated, labeled and hybridized on oligo microarray (Agilent) slides.
Experiment types
transcription profiling by array, compound treatment design, dose response design
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-10265.idf.txt
Sample and data relationshipE-MTAB-10265.sdrf.txt
Raw data (1)E-MTAB-10265.raw.1.zip
Processed data (1)E-MTAB-10265.processed.1.zip
Array designA-GEOD-10094.adf.txt
Links