E-MEXP-4001 - Transcription profiling by array of Arabidopsis thaliana wild type (Col-0) and flowering time mutants to investigate synchronized induction of flowering
Released on 1 November 2013, last updated on 17 March 2015
Synchronized induction of flowering in Arabidopsis thaliana wild type (Col-0) and flowering time mutants (soc1, agl24, fd) by shifting from short day (8 hr light, 16 hr dark; 23C; 65% rel humidity) to long day (16 hr light, 8 hr dark; 23C; 65% rel humidity) for 0, 3, 5, and 7 days. Biotinylated probes were synthesized from RNA isolated from manually disseted shoot meristems and hybridized to Affymetrix ATH1 arrays.
transcription profiling by array, co-expression, replicate design, stimulus or stress design, time series design
A quantitative and dynamic model of the Arabidopsis flowering time gene regulatory network. Leal Valentim F, Mourik Sv, Posé D, Kim MC, Schmid M, van Ham RC, Busscher M, Sanchez-Perez GF, Molenaar J, Angenent GC, Immink RG, van Dijk AD. PLoS One 10(2):e0116973 (2015), PMID:25719734