E-MEXP-3511 - Transcription profiling by array of Escherichia coli to study the persister phenotype

Status
Released on 13 October 2013, last updated on 3 June 2014
Organism
Escherichia coli
Samples (4)
Array (1)
Protocols (6)
Description
When bacteria are challenged with antimicrobials, small numbers of cells may survive treatment. These so-called persister cells are not mutants, but are a set of phenotypic variants which, after re-growth exhibit the same susceptibility properties as their progenitor populations. The presence of persisters may determine the effective therapeutic dose of antibiotics and account for the intractability of biofilm-related infections. In the current investigation, Escherichia coli persister cells were isolated without prior exposure to antimicrobials, using fluorescence-assisted cell sorting of a strain, labelled with a chromosomal green fluorescent protein marker. Levels of persistence were inversely proportional to fluorescence intensity in the gfp-tagged E. coli population, and separated persister cells showed enhanced expression of two groups of genes located in the e14 and CP4-6 cryptic prophage regions when analysed by DNA microarray.
Experiment types
transcription profiling by array, co-expression, in vivo, transcript identification
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-3511.idf.txt
Sample and data relationshipE-MEXP-3511.sdrf.txt
Raw data (1)E-MEXP-3511.raw.1.zip
Processed data (1)E-MEXP-3511.processed.1.zip
Array designA-AFFY-29.adf.txt
Links