E-MEXP-1243 - Transcription profiling by array of human prostate from patients with a previous diagnosis of Prostatic Intraepithelial Neoplasia and following consumption of high glucosinolate broccoli or peas to investigate interactions with the GSTM1 genotype
Released on 16 July 2008, last updated on 2 May 2014
Twenty-two male volunteers aged 30-70 years with a previous diagnosis of Prostatic Intraepithelial Neoplasia (PIN) were recruited by a consultant urologist at Norfolk and Norwich University Hospital NHS Trust. Ethical approval for the trial was obtained from the Norfolk Research Ethics Committee (reference 05/Q0101/9). All participants gave written, informed consent. Volunteers were excluded if they were undergoing chemopreventive therapy, were receiving testosterone replacement medication, had active infection requiring treatment, a BMI <18.5 or >35 or were diagnosed with diabetes. Subjects were allocated into a 12-month, parallel dietary intervention trial consisting of two dietary intervention groups (i) consuming 400g high glucosinolate broccoli per week or (ii) consuming 400g peas per week. Volunteers avoided foods known to contain glucosinolates for 48 hours prior to each biopsy appointment to avoid acute effects. Needle biopsy samples (4 cores) of prostate tissue were obtained by Trans Rectal Ultrasound (TRUS) guided endoscopy at baseline, six and 12 months after the start of the dietary interventions. Additionally, benign and malignant (Gleason score 7-10) tissue samples obtained by Trans Urethral Resection of the Prostate (TURP) were provided from the Partners in Cancer Research Human Tissue Bank at the Norfolk and Norwich University Hospital NHS Trust.
transcription profiling by array, co-expression, compound treatment, in vivo, physiological process, time series
Broccoli consumption interacts with GSTM1 to perturb oncogenic signalling pathways in the prostate. Traka M, Gasper AV, Melchini A, Bacon JR, Needs PW, Frost V, Chantry A, Jones AM, Ortori CA, Barrett DA, Ball RY, Mills RD, Mithen RF.