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E-GEOD-9470 - Transcriptional profiling of UV-B response of Populus alba

Status
Released on 1 December 2013, last updated on 3 June 2014
Organism
Populus alba
Samples (16)
Arrays (2)
Protocols (7)
Description
Microarray technology was used to assess transcriptome changes in poplar (Populus alba L.) under a realistic simulation of increased UV-B radiation. Plants were UV-Bbe (UV-B biologically effective radiation) supplemented with a dose of 6 kJ/m2/day for 12 hours per day and allowed to recover during the night. Poplar plants were UV-B treated using a refined controlled environment able to guarantee a realistic simulation of natural conditions, especially for light parameters such as presence of background UV-B radiation for control plants and balanced PAR/UV-A/UV-B ratio. A time course experiment was planned to look both at the rapid and delayed response of poplar to UVB; two time points after 3 h (T3h) and 30 h (6th hour of the third day of treatment, T30h) were considered. 4 independent biological replicates were analysed for each time point. Competitive hybridisations were carried out using the PICME 28K microarray. Keywords: Time course experiment, stress response Two condition experiment: UVB supplemented plants vs normal UV-B level plants. Biological replicates: 4 UVB suplemented plants, 4 control plants, two time points, one replicate per array. Dye swap between replicates.
Experiment type
transcription profiling by array 
Contacts
Giovanni Emiliani <giovanni.emiliani@unifi.it>, Donatella Paffetti, Gail Taylor, Nathaniel R Street
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-9470.idf.txt
Sample and data relationshipE-GEOD-9470.sdrf.txt
Raw data (1)E-GEOD-9470.raw.1.zip
Processed data (1)E-GEOD-9470.processed.1.zip
Array designsA-GEOD-6032.adf.txt, A-GEOD-6036.adf.txt
Links