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E-GEOD-85166 - Ccp1 homodimer mediates chromatin integrity by antagonizing CENP-A loading

Released on 4 August 2016, last updated on 5 August 2016
Schizosaccharomyces pombe
Samples (3)
Protocols (2)
CENP-A is a centromere-specific histone 3 variant essential for centromere specification. CENP-A partially replaces canonical histone H3 at the centromeres. How the particular CENP-A/H3 ratio at centromeres is precisely maintained is unknown. It also remains unclear how CENP-A is excluded from non-centromeric chromatin. Here we identify Ccp1, an uncharacterized NAP family protein in fission yeast that antagonizes CENP-A loading at both centromeric and non-centromeric regions. Like the CENP-A loading factor HJURP, Ccp1 interacts with CENP-A, and is recruited to centromeres at the end of mitosis in a Mis16-dependent manner. These data indicate that factors with opposing CENP-A loading activities are recruited to centromeres. Furthermore, Ccp1 also cooperates with H2A.Z to evict CENP-A assembled in euchromatin. Structural analyses indicate that Ccp1 forms a homodimer that is required for its anti-CENP-A loading activity. Our study establishes mechanisms for maintenance of CENP-A homeostasis at centromeres and the prevention of ectopic assembly of centromeres. Examination of cnp1 distribution in one wild type (wt) and two ccp1 mutants.
Experiment type
Fei Li
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-GEOD-85166.idf.txt
Sample and data relationshipE-GEOD-85166.sdrf.txt
Processed data (1)