Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-7819 - Transcription profiling by array of human glial cells resistant (SVGR2) and sensitive (SVG-A) to human polyomavirus JC virus infection

Status
Released on 7 November 2008, last updated on 6 October 2015
Organism
Homo sapiens
Samples (12)
Arrays (2)
Protocols (4)
Description
SVGR2 cells are glial cells which are derived from SVG-A cells. They were created by subjecting SVG-A cells to multiple rounds of lytic infection by the human polyomavirus JCV. SVGR2 cells are the cells that survived this process and are resistant to JCV infection. This experiment was designed to identify gene expression differences that may be responsible for SVGR2 resistance to JCV. Experiment Overall Design: SVG-A and SVGR2 cells are transformed cell lines. To eliminate gene expression differences that may have occurred due to genetic instability of these cell lines three subclones of each of the cell lines were created and used as a triplicate in the comparison. Total RNA was extracted from cells that had been left at 100% confluency for one day using the Qiagen RNeasy kit. cDNA was hybridized to the Affymetrix Human GeneChips U133A or U133B according to Affymetrix gudielines.
Experiment types
transcription profiling by array, cell type comparison
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7819.idf.txt
Sample and data relationshipE-GEOD-7819.sdrf.txt
Raw data (1)E-GEOD-7819.raw.1.zip
Array designsA-AFFY-33.adf.txt, A-AFFY-34.adf.txt
Links