Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-76600 - Human Calu-3 cell transcriptome response to a wild type infectious clone of H5N1 Influenza virus and mutant H5N1 viruses [miRNA]

Status
Released on 20 February 2016, last updated on 27 February 2016
Organism
Homo sapiens
Samples (27)
Array (1)
Protocols (7)
Description
The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in human Calu-3 cells infected with wild-type A/Vietnam/1203/2004 (H5N1; 'VN1203'; derived by reverse genetics), PB2-K627E (627K is a mammalian-adapting mutation that increases polymerase replicatve activity; '627E' is typically found in avian species), and NS1-trunc124 (this 'NS1' protein has a 90 amino acid truncation at the C-terminus). Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 2 days before infection. Prior to infection, washed cells 2 times with PBS, and then infected with a multiplicity of infection of 1. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 7, 12, and 24 hr post-infection.
Experiment type
transcription profiling by array 
Contacts
Yoshihiro Kawaoka <kawaokay@vetmed.wisc.edu>, Amie J Eisfeld, Katrina M Waters, Kelly Stratton, Kevin Walters
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-76600.idf.txt
Sample and data relationshipE-GEOD-76600.sdrf.txt
Raw data (1)E-GEOD-76600.raw.1.zip
Processed data (1)E-GEOD-76600.processed.1.zip
Additional data (1)E-GEOD-76600.additional.1.zip
Array designA-GEOD-19730.adf.txt
Links