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E-GEOD-74371 - GATA-1 and heme regulate the erythroid cell transcriptome

Status
Released on 7 January 2016, last updated on 17 January 2016
Organism
Mus musculus
Samples (12)
Protocols (4)
Description
Alas2 gene encodes the rate-limiting enzyme in heme biosynthesis. CRISPR/Cas9-mediated ablation of two Alas2 intronic cis-elements strongly reduced GATA-1-induced Alas2 transcription, heme biosynthesis, and GATA-1 regulation of other vital constituents of the erythroid cell transcriptome. Bypassing Alas2 function in Alas2 cis-element-mutant (double mutant) cells by providing its catalytic product 5-aminolevulinic acid (5-ALA) rescued heme biosynthesis and the GATA-1-dependent genetic network. We discovered a GATA factor- and heme-dependent circuit that establishes the erythroid cell transcriptome. G1E-ER-GATA-1 WT and double mutant cells were examined. Untreated WT, beta-estradiol-treated WT, beta-estradiol-treated double-mutant, and beta-estradiol/5-ALA-treated double-mutant cells were subjected to RNA-seq.
Experiment type
RNA-seq of coding RNA 
Contacts
Kyle Hewitt <KJHEWITT@WISC.EDU>, Colin N Dewey, Emery H Bresnick, Nobuyuki Tanimura
Citation
Mechanism governing heme synthesis reveals a GATA factor/heme circuit that controls differentiation. Tanimura N, Miller E, Igarashi K, Yang D, Burstyn JN, Dewey CN, Bresnick EH. , PMID:26698166
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-74371.idf.txt
Sample and data relationshipE-GEOD-74371.sdrf.txt
Processed data (1)E-GEOD-74371.processed.1.zip
Links