Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-70059 - Whole blood transcriptome analysis of Mycoplasma mycoides subsp, mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation
Released on 20 June 2015, last updated on 27 June 2015
In order to assess the use of whole blood transcriptome analysis to study cattle-Mmm interactions, we performed this study on 6 cattle, comparing the blood transcriptome before infection with the beginning of the clinical signs. Statistical analysis enabled Mmm-infected animals to be clearly distinguished from healthy ones. Functionnal analysis revealed that 680 genes were differentially expressed, confirming previous results as immunosuppression. Surprisingly, whoole blood transcriptome analysis didn't reflect inflammation, a highly characteristic local sign of these disease. This absence on inflammation signs results were confirmed by RT-qPCR. Whole blood gene expression was measured for each of 6 animals before infection and at the beginning of the clinical signs of CBPP. A paired sample statistical analysis was performed to identify diferentially expressed genes between healthy and infected status.
transcription profiling by array
Rodrigues Valérie <firstname.lastname@example.org>, Carinne Puech, François Thiaucourt, Hezron Wesonga, Lucia Manso-Silvan, Philippe Holzmuller, Valérie Rodrigues