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E-GEOD-70059 - Whole blood transcriptome analysis of Mycoplasma mycoides subsp, mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation

Status
Released on 20 June 2015, last updated on 27 June 2015
Organism
Bos taurus
Samples (12)
Array (1)
Protocols (7)
Description
In order to assess the use of whole blood transcriptome analysis to study cattle-Mmm interactions, we performed this study on 6 cattle, comparing the blood transcriptome before infection with the beginning of the clinical signs. Statistical analysis enabled Mmm-infected animals to be clearly distinguished from healthy ones. Functionnal analysis revealed that 680 genes were differentially expressed, confirming previous results as immunosuppression. Surprisingly, whoole blood transcriptome analysis didn't reflect inflammation, a highly characteristic local sign of these disease. This absence on inflammation signs results were confirmed by RT-qPCR. Whole blood gene expression was measured for each of 6 animals before infection and at the beginning of the clinical signs of CBPP. A paired sample statistical analysis was performed to identify diferentially expressed genes between healthy and infected status.
Experiment type
transcription profiling by array 
Contacts
Rodrigues Valérie <valerie.rodrigues@cirad.fr>, Carinne Puech, François Thiaucourt, Hezron Wesonga, Lucia Manso-Silvan, Philippe Holzmuller, Valérie Rodrigues
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-70059.idf.txt
Sample and data relationshipE-GEOD-70059.sdrf.txt
Raw data (1)E-GEOD-70059.raw.1.zip
Processed data (1)E-GEOD-70059.processed.1.zip
Array designA-GEOD-11649.adf.txt
Links