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E-GEOD-67923 - Comparison of gene expression changes in AML cells after SMARCA4, SMARCD2 or DPF2 knock-down

Status
Released on 12 July 2015, last updated on 19 August 2015
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (7)
Description
We explored the role of SMARCA4 and the two Brahma associated factors SMARCD2 and DPF2 in leukaemia. We observed the selective requirement for these factors for leukemic cell expansion, as well as extended survival of mice transplanted with leukaemic cells with reduced expression of these genes. Gene expression profiling revealed largely similar alterations with the down-regulation of each of these three factors, suggesting a concerted function in transformed blood cells. These changes included loss of pluripotency-associated signature but did not correlate with c-MYC down-regulation. Human acute monocytic leukemia cells (THP-1, ACC 16) were lentivirally transduced with pLKO-puro vectors carrying shRNAs against SMARCA4, SMARCD2 or DPF2. As a negative control, THP-1 cells were transduced with pLKO-puro-shScr (Scrambled). All samples were prepared as biological triplicates.
Experiment type
transcription profiling by array 
Contacts
Kristian Helin <kristian.helin@bric.ku.dk>, Patrycja Sroczynska, V A Cruickshank
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-67923.idf.txt
Sample and data relationshipE-GEOD-67923.sdrf.txt
Raw data (1)E-GEOD-67923.raw.1.zip
Processed data (1)E-GEOD-67923.processed.1.zip
Array designA-GEOD-16699.adf.txt
Links