Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-6690 - Transcription profiling of mouse ANA-1 macrophages infected with Chlamydia pneumoniae
Submitted on 9 January 2007, released on 15 June 2008, last updated on 27 March 2012
This experiment is an additional experiment to GSE6688. Mouse macrophages (ANA-1 cells) were infected in vitro with C. pneumoniae with a M.O.I. of 10. Twenty two genes were significantly upregulated. Examples of the most upregulated genes in mouse macrophages after C. pneumoniae infection are serum amyloid A3 (saa3), a protein that is mainly produced by activated macrophages during tissue injury or inflammation, MIP-2 (cxcl2) and irg1. Expression levels of all genes induced by C. pneumoniae in macrophages in vitro correlated with the results obtained from infected lungs from wild type mice (GSE6688), suggesting that this cell type participates in host defense in vivo against C. pneumoniae. Experiment Overall Design: ANA-1 macrophages were infected with Chlamydia pneumoniae or left untreated. After 8h total RNA was extracted. Two biological replicates were performed resulting in 4 arrays in total
transcription profiling by array, disease state
MyD88-dependent changes in the pulmonary transcriptome after infection with Chlamydia pneumoniae. Nuria Rodríguez, Jörg Mages, Harald Dietrich, Nina Wantia, Hermann Wagner, Roland Lang, Thomas Miethke. Physiol Genomics 30(2):134-45 (2007)