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E-GEOD-66082 - Widespread association between YAP/TAZ/TEAD and AP-1 at enhancers drives oncogenic growth [gene expression]

Status
Released on 3 August 2015, last updated on 19 August 2015
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (7)
Description
The goal of this study was to identify YAP/TAZ direct transcriptional targets and transcriptional partners, through ChIP-sequencing and gene expression profiling. Keywords: Expression profiling by array MDA-MB-231 cells growing at low confluence in standard growth conditions were transfected with the indicated siRNAs (Dupont et al., Nature 2011). The following day, growth medium was renewed and cells were incubated for 24 more hours, and then harvested for total RNA extraction. 4 independent biological replicates were plated, transfected and harvested in parallel for each siRNA. Raw .CEL files are the same as in GSE59230 (Enzo et al., EMBO J 2015), but gene expression levels have been quantified using Brainarray HGU133Plus2_Hs_ENTREZG_v17 custom chip definition files.
Experiment types
transcription profiling by array, unknown experiment type 
Contacts
Francesca Zanconato, Mattia Forcato, Michelangelo Cordenonsi, Silvio Bicciato, Stefano Piccolo
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-66082.idf.txt
Sample and data relationshipE-GEOD-66082.sdrf.txt
Raw data (1)E-GEOD-66082.raw.1.zip
Processed data (1)E-GEOD-66082.processed.1.zip
Array designA-GEOD-17811.adf.txt
Links