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E-GEOD-64686 - Expression data from the inducible expression of EWS-FLI1 (EF) in embryoid bodies

Status
Released on 31 December 2015, last updated on 1 January 2016
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (6)
Description
Oncogenic transformation in Ewing sarcoma tumors is driven by the fusion oncogene EWS-FLI1. The inducible expression of EWS-FLI1 (EF) in embryoid bodies, or collections of differentiating stem cells, generates cells with properties of Ewing sarcoma tumors, including characteristics of transformation. These cell lines exhibit anchorage-independent growth, a lack of contact inhibition and a strong Ewing sarcoma gene expression signature. These cells also demonstrate a requirement for the persistent expression of EWS-FLI1 for cell survival and growth. We used microarrays to detail the effects of doxycycline-inducible expression of EWS-FLI1 on the gene expression signature of cells derived from differentiating stem cells, or embryoid bodies. Triplicate biological replicates were collected and analyzed.
Experiment type
transcription profiling by array 
Contacts
David Jon Gordon <david-j-gordon@uiowa.edu>, David Gordon, David Pellman, Mona Motwani
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-64686.idf.txt
Sample and data relationshipE-GEOD-64686.sdrf.txt
Raw data (1)E-GEOD-64686.raw.1.zip
Processed data (1)E-GEOD-64686.processed.1.zip
Array designA-AFFY-44.adf.txt
Links