Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-64057 - Rapid functional annotation of native enhancers in embryonic stem cells with a Neisseria meningitidis CRISPR-Cas9 system (BeadChip)

Status
Released on 3 March 2015, last updated on 7 March 2015
Organism
Mus musculus
Samples (16)
Array (1)
Protocols (7)
Description
Gene regulatory elements such as enhancers have profound effects on cellular function, health, and disease. Our understanding of mammalian enhancer function is limited by the lack of a technology that would allow for a rapid and thorough test of their cell type-specific function. Here, we describe a novel Cas9-effector system that enables rapid testing and functional annotation of native enhancers in embryonic stem cells. Total RNA obtained from R26 dCas9-effector mESC lines after viral delivery of sgRNAs directed against proximal promoter or enhancer regions
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-64057.idf.txt
Sample and data relationshipE-GEOD-64057.sdrf.txt
Processed data (1)E-GEOD-64057.processed.1.zip
Additional data (1)E-GEOD-64057.additional.1.zip
Array designA-MEXP-1174.adf.txt
Links