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E-GEOD-63662 - GM-CSF induced gene-regulation in human monocytes

Status
Released on 27 November 2014, last updated on 28 November 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
Human and murine studies showed that granulocyte macrophage colony-stimulating factor (GM-CSF) exerts beneficial effects in intestinal inflammation. To explore whether GM-CSF mediates its effects via monocytes, we analyzed effects of GM-CSF on monocytes in vitro and assessed the immunomodulatory potential of GM-CSF-activated monocytes (GMaM). We used microarray technology and functional assays to characterize GMaM in vitro and used a mouse model of colitis to study GMaM functions in vivo. Peripheral blood monocytes where cultured 16 h with media containing AB serum (control monocytes) or media containing 10 ng/mL GM-CSF and AB serum (GM-CSF activated monocytes). In three independent experiments, total RNA from GMaM and control monocytes was isolated and processed for microarray hybridization.
Experiment type
transcription profiling by array 
Contacts
Toni Weinhage <weinhage@uni-muenster.de>, Anne Brockhausen, David Schwarzmaier, Dirk Foell, Dominik Bettenworth, Georg Varga, Jan Däbritz, Jan M Ehrchen, Johannes Roth, Karoline Walscheid, Markus Brückner, Matthias Ross, Timo Wirth
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-63662.idf.txt
Sample and data relationshipE-GEOD-63662.sdrf.txt
Raw data (1)E-GEOD-63662.raw.1.zip
Processed data (1)E-GEOD-63662.processed.1.zip
Array designA-AFFY-44.adf.txt
Links