Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-63662 - GM-CSF induced gene-regulation in human monocytes
Released on 27 November 2014, last updated on 28 November 2014
Human and murine studies showed that granulocyte macrophage colony-stimulating factor (GM-CSF) exerts beneficial effects in intestinal inflammation. To explore whether GM-CSF mediates its effects via monocytes, we analyzed effects of GM-CSF on monocytes in vitro and assessed the immunomodulatory potential of GM-CSF-activated monocytes (GMaM). We used microarray technology and functional assays to characterize GMaM in vitro and used a mouse model of colitis to study GMaM functions in vivo. Peripheral blood monocytes where cultured 16 h with media containing AB serum (control monocytes) or media containing 10 ng/mL GM-CSF and AB serum (GM-CSF activated monocytes). In three independent experiments, total RNA from GMaM and control monocytes was isolated and processed for microarray hybridization.
transcription profiling by array
Toni Weinhage <email@example.com>, Anne Brockhausen, David Schwarzmaier, Dirk Foell, Dominik Bettenworth, Georg Varga, Jan Däbritz, Jan M Ehrchen, Johannes Roth, Karoline Walscheid, Markus Brückner, Matthias Ross, Timo Wirth