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E-GEOD-6236 - Transcription profiling of human circulating blood reticulocytes from adult and cord blood to provide a robust description of genes transcribed at the final stages of erythroblast maturation

Status
Released on 1 November 2007, last updated on 27 March 2012
Organism
Homo sapiens
Samples (56)
Arrays (2)
Protocols (7)
Description
RNA from circulating blood reticulocytes was utilized to provide a robust description of genes transcribed at the final stages of erythroblast maturation. After depletion of leukocytes and platelets, Affymetrix HG-U133 arrays were hybridized with probe from total RNA isolated from blood sampled from 14 umbilical cords and 14 healthy adult humans. Experiment Overall Design: Peripheral blood from 14 normal adult and 14 human umbilical cord were purified to obtain packed red blood cells and their purity was assessed by CELL-DYN4000. The contaminated WBC count was less than 1 out of 1 million cells. Total RNA from purified reticulocytes included in the purified red blood cells was extracted, labeled, and hybridized onto Affymetrix HG-U133 A and B arrays.
Experiment types
transcription profiling by array, co-expression, development or differentiation
Contact
Citation
The human reticulocyte transcriptome. Sung-Ho Goh, Matthew Josleyn, Y Terry Lee, Robert L Danner, Robert B Gherman, Maggie C Cam, Jeffery L Miller.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-6236.idf.txt
Sample and data relationshipE-GEOD-6236.sdrf.txt
Raw data (2)E-GEOD-6236.raw.1.zip, E-GEOD-6236.raw.2.zip
Processed data (1)E-GEOD-6236.processed.1.zip
Array designsA-AFFY-33.adf.txt, A-AFFY-34.adf.txt
R ExpressionSetE-GEOD-6236.eSet.r
Links