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E-GEOD-61859 - Defined conditions for the isolation and expansion of basal prostate stem cells of mouse and human origin [human]

Status
Released on 20 February 2015, last updated on 28 February 2015
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (5)
Description
Isolation and culture of primary prostate epithelial stem/progenitor cells (PESC) has been proven difficult and ineffective. Here we present methods to grow and expand both murine and human basal PESCs long-term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin-Sca1+ CD49f+Trop2high phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin-CD49f+Trop2high PESCs. The gene expression profiles of expanded basal PESCs show similarities to ES cells and Lamin B1 and PRDX1 were identified as novel PESC markers. If transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules demonstrating their stem cell activity in vivo. The novel methods will facilitate the cellular, molecular and genomic characterization of normal and pathologic prostate glands of mouse and human origin. Human prostate cells derived from two individual patients were seperately cultured under spheroid and adherent stem cell conditions and subsequently used for RNA extraction
Experiment type
transcription profiling by array 
Contacts
Eisen Christian <geo@ncbi.nlm.nih.gov>, Christian Eisen, Thomas Höfner
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-61859.idf.txt
Sample and data relationshipE-GEOD-61859.sdrf.txt
Processed data (1)E-GEOD-61859.processed.1.zip
Additional data (1)E-GEOD-61859.additional.1.zip
Array designA-GEOD-10558.adf.txt
Links