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E-GEOD-61351 - RNA-seq Analysis of Mineralizing IDG-SW3 Cultures with Mature Osteocyte Characteristics

Status
Released on 3 March 2015, last updated on 12 March 2015
Organism
Mus musculus
Samples (6)
Protocols (4)
Description
We differentiated the murine IDG-SW3 cell line for 28 days until the cells displayed a mature osteocyte-like phenotype. Triplicate cultures of the IDG-SW3 cells were then treated with 50nM PTH (1-34) or vehicle control (PBS) for 24 hours. RNA was harvested from the cultures and used to perform RNA Seq analysis. We found that many genes previously shown to be markers of the osteocyte phenotype were strongly downregulated in response to PTH treatment. Furthermore, we found that genes known to inhibit cell motility were downregulated in response to PTH, whereas genes promoting motility were upregulated. This corresponds to the increased cell motility observed in PTH-treated IDG-SW3 cell cultures. Therefore, PTH induces a switch in mature IDG-SW3 cells from a osteocyte-like cell to a more motile phenotype. RNA expression profiles of control and PTH-treated 28 day differentiated IDG-SW3 cells.
Experiment type
RNA-seq of coding RNA 
Contacts
stephen e harris <harris@uthscsa.edu>, Lynda L Bonewald, Mathew Prideaux, Stephen E Harris
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-61351.idf.txt
Sample and data relationshipE-GEOD-61351.sdrf.txt
Processed data (1)E-GEOD-61351.processed.1.zip
Additional data (1)E-GEOD-61351.additional.1.zip
Links