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E-GEOD-60451 - Sternal cartilage microarray (WT vs. TIMPless)

Status
Released on 31 August 2015, last updated on 6 September 2015
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
Timps are natural metalloproteinase inhibitors that direct the cell microenvironment in health and disease, yet the essential requirement of this gene family in mammals is unknown. We generated quadruple Timp deficient mice lacking Timp1, Timp2, Timp3 and Timp4 (TIMPless) and found that Timp function is essential for postnatal lifespan, lung form and function and skeletogenesis. TIMPless mice survive embryogenesis but develop pervasive skeletal aberrations characterized by axial cartilage overgrowth and growth plate closure in long bones. We performed microarray analysis to identify signaling pathways affected by the loss of the entire Timp family in sternal cartilage. Cartilage, excluding the xiphoid process, was macrodissected from the sternums of 4-week old WT and TIMPless mice (n=6/genotype).
Experiment type
transcription profiling by array 
Contacts
Rama Khokha <rkhokha@uhnres.utoronto.ca>, Alison Aiken, Yu-Jia Shiah
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-60451.idf.txt
Sample and data relationshipE-GEOD-60451.sdrf.txt
Raw data (1)E-GEOD-60451.raw.1.zip
Processed data (1)E-GEOD-60451.processed.1.zip
Array designA-GEOD-16570.adf.txt
Links