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E-GEOD-59786 - Human IFNG+/- Treg and Tconv transcriptome

Status
Released on 1 May 2015, last updated on 10 May 2015
Organism
Homo sapiens
Samples (40)
Array (1)
Protocols (7)
Description
Microarray used to detail the global gene transcription underlying sorted IFNg+ and IFNg- Tregs (CD4+CD25+CD127lo) and Tconv (CD4+CD25-CD127+) for fresh (unexpanded) and 14 day expanded cells from human blood. Treg and Tconv were FACS isolated from five healthy subjects (median age of 26, range 22-30). Sorted cells were separated into two groups: the first group was stimulated for 4 hours with PMA/ionomycin and labeled with the IFNg cytokine capture kit (Miltenyi Biotech) followed by FACS isolation of IFNg- and IFNg+ populations. The second set was expanded to day 14 prior to reactivation and cytokine cell capture. For each IFNg sorted population, cells were pelleted and flash frozen before RNA isolation and processing.
Experiment type
transcription profiling by array 
Contacts
GAURAV CHOPRA <gchopra@diabetes.ucsf.edu>, David V Schack, Gaurav Chopra, Jeffrey A Bluestone, Mahesh Yadav, Maryann Whitley, Stephanie A McClymont, Ying Zhang, Yizheng Li
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-59786.idf.txt
Sample and data relationshipE-GEOD-59786.sdrf.txt
Raw data (2)E-GEOD-59786.raw.1.zip, E-GEOD-59786.raw.2.zip
Processed data (1)E-GEOD-59786.processed.1.zip
Array designA-AFFY-44.adf.txt
Links