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E-GEOD-59285 - The G Protein-coupled Receptor P2Y14 Influences Insulin Release and Smooth Muscle Function in Mice

Status
Released on 11 July 2014, last updated on 8 December 2014
Organism
Mus musculus
Samples (20)
Protocols (3)
Description
UDP-sugars were identified as extracellular signaling molecules, assigning a new function to these compounds in addition to their well defined role in intracellular substrate metabolism and storage. Previously regarded as an orphan receptor, the G protein-coupled receptor (GPCR) P2Y14 (GPR105) was found to bind extracellular UDP and UDP-sugars. Little is known about the physiological functions of this GPCR. To study its physiological role we used a gene-deficient (KO) mouse strain expressing the bacterial LacZ reporter gene to monitor the physiological expression pattern of P2Y14. We found that P2Y14 is mainly expressed in pancreas and salivary glands and in subpopulations of smooth muscle cells of the gastrointestinal tract, blood vessels, lung and uterus. Among other phenotypical differences KO mice showed a significantly impaired glucose tolerance following oral and intraperitoneal glucose application. An unchanged insulin tolerance suggested altered pancreatic islet function. Transcriptome analysis of pancreatic islets showed that P2Y14 deficiency significantly changed expression of components involved in insulin secretion. Insulin secretion tests revealed a reduced insulin release from P2Y14-deficient islets highlighting P2Y14 as a new modulator of proper insulin secretion. 10 samples from pancreatic islets isolated from wildtype mice; 10 samples from pancreatic islets isolated from P2Y14-knockout mice
Experiment type
RNA-seq of coding RNA 
Contacts
Torsten Schöneberg <torsten.schoeneberg@medizin.uni-leipzig.de>, Albert Ricken, Angela Schulz, Diana Le Duc, Helga Pfannkuche, Jaroslawna Meister, Joachim Thiery, Johannes Grosse, Ralph Burkhardt, Tobias Polte, Torsten Schoneberg
Citation
The G Protein-coupled Receptor P2Y14 Influences Insulin Release and Smooth Muscle Function in Mice. Meister J, Le Duc D, Ricken A, Burkhardt R, Thiery J, Pfannkuche H, Polte T, Grosse J, Schoneberg T, Schulz A. , PMID:24993824
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-59285.idf.txt
Sample and data relationshipE-GEOD-59285.sdrf.txt
Processed data (1)E-GEOD-59285.processed.1.zip
Additional data (1)E-GEOD-59285.additional.1.zip
Links