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E-GEOD-5922 - Transcription profiling of human primary CD34+ cells undergoing granulocytic differentiation cultured under 5% and 20% O2 levels

Status
Released on 14 June 2008, last updated on 2 May 2014
Organism
Homo sapiens
Samples (126)
Array (1)
Protocols (22)
Description
Little is known about the global transcriptional program underlying granulocytic (G) commitment, differentiation and maturation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of G differentiation of human CD34-positive hematopoietic stem and progenitor cells cultured with interleukin-3, interleukin-6 granulocyte colony stimulating factor and stem cell factor. The goal this study was to identify genes involved in the various facets of G differentiation including commitment, expansion, differentiation and functional capacity. This SuperSeries is composed of the following subset Series:; GSE5917: Temporal expression profile of granulocytic differentiation of primary CD34+ cells cultured under 20% O2 levels; GSE5918: Temporal expression profile of granulocytic differentiation of primary CD34+ cells cultured under 5% O2 levels; GSE6792: The transcriptional patterns of mature normal PB neutrophils (granulocytes) was examined and used as a control against which the transcriptional programs of cultured granulocytes and granulocytic cells lines can be compared. Experiment Overall Design: Refer to individual Series
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-5922.idf.txt
Sample and data relationshipE-GEOD-5922.sdrf.txt
Processed data (1)E-GEOD-5922.processed.1.zip
Array designA-AGIL-9.adf.txt
Links