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E-GEOD-59202 - Fluorochrome-based definition of naturally occurring Foxp3+ regulatory T cells of intra- and extrathymic origin

Status
Released on 9 July 2014, last updated on 14 July 2014
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (7)
Description
Here, we report on experiments in double-transgenic mice, in which RFP is expressed in all Foxp3+ Treg cells, whereas Foxp3-dependent GFP expression is exclusively confined to intrathymically induced Foxp3+ Treg cells. This novel molecular genetic tool enabled us to faithfully track and characterize naturally induced Treg cells of intrathymic (RFP+GFP+) and extrathymic (RFP+GFP−) origin in otherwise unmanipulated mice. These experiments directly demonstrate that extrathymically induced Treg cells substantially contribute to the overall pool of mature Foxp3+ Treg cells residing in peripheral lymphoid tissues of steady-state mice. Furthermore, we provide evidence that intra- and extrathymically induced Foxp3+ Treg cells represent distinct phenotypic and functional sublineages. CD4+CD25+ RFP+GFP- and CD4+CD25+ RFP+GFP+ T cells from pooled lymph nodes and pooled spleens of 20 mice were FACS sorted for RNA extraction and hybridization on Affymetrix microarrays in duplicates.
Experiment type
transcription profiling by array 
Contacts
Sonja Schallenberg <geo@ncbi.nlm.nih.gov>, Cathleen Petzold, Karsten Kretschmer, Robert Geffers
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-59202.idf.txt
Sample and data relationshipE-GEOD-59202.sdrf.txt
Raw data (1)E-GEOD-59202.raw.1.zip
Processed data (1)E-GEOD-59202.processed.1.zip
Additional data (1)E-GEOD-59202.additional.1.zip
Array designA-AFFY-45.adf.txt
Links