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E-GEOD-58096 - The transcriptomic responses of THP1 human monocyte-like cells expressing SP110b to interferon gamma stimulation

Status
Released on 18 May 2015, last updated on 24 May 2015
Organism
Homo sapiens
Samples (15)
Array (1)
Protocols (6)
Description
SP110b is an interferon (IFN)-induced nuclear protein and may function as a transcriptional co-activator/repressor. IFNγ activates monocytes/macrophages thereby mediating inflammation. However, uncontrolled activation induces monocyte/macrophage cell death, which may cause immunopathology. We have demonstrated that SP110b expression prevented IFNγ-mediated monocyte/macrophage cell death. To explore the molecular mechanisms by which SP110b suppresses IFNγ-induced cell death, we performed a genome-wide microarray analysis to identify genetic determinants associated with IFNγ-induced cell death and regulated by SP110b. We sought to identify genetic determinants associated with IFNγ-induced cell death and regulated by SP110b. To that end, THP1 human monocyte-like cells that could be induced by doxycycline (Dox) to over-express SP110b (THP1-SP110b) were generated and 5 experimental groups of THP1-SP110b cells were harvested for RNA extraction and hybridization on Affymetrix microarrays. The 5 groups are as follows: untreated THP1-SP110b cells as control (CON), cells treated with IFNγ for 2 days (IFN_2D), cells treated with Dox plus IFNγ for 2 days (DoxIFN_2D), cells treated with IFNγ for 4 days (IFN_4D), and cells treated with Dox plus IFNγ for 4 days (DoxIFN_4D).
Experiment type
transcription profiling by array 
Contacts
Bo-Shiun Yan, Jia-Shiun Leu
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-58096.idf.txt
Sample and data relationshipE-GEOD-58096.sdrf.txt
Raw data (1)E-GEOD-58096.raw.1.zip
Processed data (1)E-GEOD-58096.processed.1.zip
Array designA-AFFY-44.adf.txt
Links