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E-GEOD-55723 - Modulation of gene expression induced by cylindrospermopsin in human intestinal Caco-2 cells

Status
Released on 11 March 2014, last updated on 3 June 2014
Organism
Homo sapiens
Samples (11)
Array (1)
Protocols (7)
Description
Cylindrospermopsin (CYN) is a cyanotoxin that has been recognised as an emerging potential public health risk. Although CYN toxicity has been demonstrated, the mechanisms involved have not been fully characterised. To identify some key pathways related to this toxicity, we studied the transcriptomic profile of human intestinal Caco-2 cells exposed to a sub-toxic concentration of CYN (1.6 µM for 24hrs) using a non-targeted approach. CYN was shown to modulate different biological functions which were related to growth arrest (with down-regulation of cdkn1a and uhrf1 genes), and DNA recombination and repair (with up-regulation of aptx and pms2 genes). Our main results reported an increased expression of some histone-modifying enzymes (histone acetyl and methyltransferases MYST1, KAT5 and EHMT2) involved in chromatin remodelling, which is essential for initiating transcription. We also detected greater levels of acetylated histone H2A (Lys5) and dimethylated histone H3 (Lys4), two products of these enzymes. In conclusion, CYN overexpressed proteins involved in DNA damage repair and transcription, including modifications of nucleosomal histones. Our results highlighted some new cell processes induced by CYN. Modulation of gene expression in differentiated Caco-2 cells was measured at 24 hours after exposure to doses of 0 and 1.6 µM of cylindrospermopsin. Six independent experiments were performed, but only 11 samples (5 controls and 6 exposed to cylindrospermopsin) had an RNA quality indicator ≥ 9 and were used for the microarray experiments.
Experiment type
transcription profiling by array 
Contacts
Antoine Huguet
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-55723.idf.txt
Sample and data relationshipE-GEOD-55723.sdrf.txt
Raw data (1)E-GEOD-55723.raw.1.zip
Processed data (1)E-GEOD-55723.processed.1.zip
Array designA-AGIL-28.adf.txt
Links