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E-GEOD-55515 - MicroRNA-223 and miR-143 are important systemic biomarkers for disease activity in psoriasis

Status
Released on 10 June 2014, last updated on 14 June 2014
Organism
Homo sapiens
Samples (34)
Array (1)
Protocols (4)
Description
Background: Psoriasis is a systemic inflammatory skin disease. MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that recently have been found in the blood to be relevant as disease biomarkers. Objective: We aimed to explore miRNAs potential as blood biomarkers for psoriasis. Methods: Using microarray and quantitative real-time PCR we measured the global miRNA expression in whole blood, plasma and peripheral blood mononuclear cells (PBMCs) from patients with psoriasis and healthy controls. Results: We identified several deregulated miRNAs in the blood from patients with psoriasis including miR-223 and miR-143 which were found to be significantly upregulated in the PBMCs from patients with psoriasis compared with healthy controls (FCH=1.63, P<0.01; FCH=2.18, P<0.01, respectively). In addition, miR-223 and miR-143 significantly correlated with the PASI score (r = 0.46, P<0.05; r=0.55, P<0.02, respectively). Receiver-operating characteristic analysis (ROC) showed that miR-223 and -143 have the potential to distinguish between psoriasis and healthy controls (miR-223: Area under the curve (AUC) = 0.80, miR-143: AUC = 0.75). Interestingly, after 3-5 weeks of treatment with methotrexate following a significant decrease in psoriasis severity, miR-223 and miR-143 were significantly downregulated in the PBMCs from patients with psoriasis. Conclusion: We suggest that changes in the miR-223 and miR-143 expressions in PBMCs from patients with psoriasis may serve as novel biomarkers for disease activity in psoriasis; however, further investigations are warranted to clarify their specific roles. In the present study, we compared the global miRNA expression profile between whole blood samples obtained from 24 patients with psoriasis (5 samples were excluded due to poor quality control) compared with 15 healthy controls.
Experiment type
transcription profiling by array 
Contacts
Marianne Bengtson Løvendorf <mabelo01@geh.regionh.dk>, John R Zibert, Lone Skov, Mads A Røpke, Marianne B Løvendorf, Mette Gyldenløve
Citation
MicroRNA-223 and miR-143 are important systemic biomarkers for disease activity in psoriasis. L�vendorf MB, Zibert JR, Gyldenl�ve M, R�pke MA, Skov L. , PMID:24909097
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-55515.idf.txt
Sample and data relationshipE-GEOD-55515.sdrf.txt
Raw data (1)E-GEOD-55515.raw.1.zip
Additional data (1)E-GEOD-55515.additional.1.zip
Array designA-GEOD-11241.adf.txt
Links