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E-GEOD-55033 - MicroRNA-15/16 Antagonizes c-Myb to Control Natural Killer Cell Maturation

Status
Released on 3 September 2015, last updated on 4 September 2015
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
NK cells develop in the bone marrow and complete their maturation in peripheral organs, but the molecular events controlling maturation are incompletely understood. Utilizing an NK cell-specific miR-15/16 deficient genetic model (15aKO), we identified a critical role for miR-15/16 family microRNAs in the normal maturation of NK cells in vivo, with a specific reduction in mature CD11b+CD27- NK cells in multiple tissues. The mechanism responsible was a block in differentiation, since accelerated NK cell death was not evident, and earlier intermediates of NK cell maturation were expanded. Further, we identified Myb as a direct target of miR-15/16 in NK cells, with Myb expression increased in immature 15aKO NK cells. Following adoptive transfer, immature 15aKO NK cells exhibited defective maturation, which was rescued by ectopic miR-15/16 expression or Myb knockdown. Moreover, Myb overexpression resulted in defective NK cell maturation. Thus, miR-15/16 regulation of Myb controls the normal NK cell maturation program. 3 technical replicates each of CD27+ 15a/16-1FKO NK cells, and CD27+ Ctrl NK cells
Experiment type
transcription profiling by array 
Contacts
Citation
MicroRNA-15/16 Antagonizes Myb To Control NK Cell Maturation. Sullivan RP, Leong JW, Schneider SE, Ireland AR, Berrien-Elliott MM, Singh A, Schappe T, Jewell BA, Sexl V, Fehniger TA.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-55033.idf.txt
Sample and data relationshipE-GEOD-55033.sdrf.txt
Raw data (1)E-GEOD-55033.raw.1.zip
Processed data (1)E-GEOD-55033.processed.1.zip
Array designA-AFFY-130.adf.txt
Links