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E-GEOD-52327 - Expression data of ALDH+ breast cancer cells

Status
Released on 14 November 2013, last updated on 28 August 2015
Organism
Homo sapiens
Samples (16)
Array (1)
Protocols (5)
Description
It has been suggested that breast cancers are driven and maintained by a cellular subpopulation with stem cell properties. These breast cancer stem cells (BCSCs) mediate metastasis and by virtue of their resistance to radiation and chemotherapy, contribute to relapse. Although several BCSC markers have been described, it is unclear whether these markers identify the same or independent BCSC populations. Based on established breast cancer cell lines, as well as primary tumor samples and xenografts, we show that BCSCs exist in distinct mesenchymal-like (epithelial-mesenchymal transition, EMT) and epithelial-like (mesenchymal-epithelial transition, MET) states characterized by expression of distinct markers, proliferative capacity and invasive characteristics. The gene expression profiles of mesenchymal-like and epithelial-like BCSCs are remarkably similar across the different molecular subtypes of breast cancer and resemble those of distinct basal and luminal stem cells found in the normal breast. We propose that the plasticity of BCSCs allowing them to transition between EMT- and MET-like states endows these cells with the capacity for tissue invasion, dissemination and growth at metastatic sites. Breast cancer cells from patient were sorted using flow cytometry to select for cells that were ALDH+. Gene expression profiles of these cells were compared with profiles of ALDH- cells.
Experiment type
transcription profiling by array 
Contacts
yang CONG <ycong@tmhs.org>, Christophe Ginestier, Daniel Birnbaum, Emmanuelle Charafe-Jauffret
Citation
Breast cancer stem cells transition between epithelial and mesenchymal states reflective of their normal counterparts. Liu S, Cong Y, Wang D, Sun Y, Deng L, Liu Y, Martin-Trevino R, Shang L, McDermott SP, Landis MD, Hong S, Adams A, D'Angelo R, Ginestier C, Charafe-Jauffret E, Clouthier SG, Birnbaum D, Wong ST, Zhan M, Chang JC, Wicha MS.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-52327.idf.txt
Sample and data relationshipE-GEOD-52327.sdrf.txt
Raw data (1)E-GEOD-52327.raw.1.zip
Processed data (1)E-GEOD-52327.processed.1.zip
Array designA-AFFY-44.adf.txt
Links