Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-51540 - Effects of TNF-alpha blocking in sorted Th17 cells from co-cultures of human CD4-positive and CD14-positive cells
Released on 1 November 2013, last updated on 18 November 2013
Human CD4+ T cells and CD14+ monocytes from healthy donors were co-cultured with anti-CD3 for three days in the presence or absence of TNF-alpha mAb (Adalimumab). Classical Th17 cells (Th17) or those generated in the presence of the inhibitor (iTh17) were then sorted and analyzed by full transcriptome microarray analysis. A total of 18 samples consisting of paired samples from 9 healthy volunteers were compared. Sorted Th17 cells from co-cultures containing either Adalimumab (1 microgram/ml) or not were obtained from each of the donors, and compared by full transcriptome profiling.
transcription profiling by array
Klaus Stensgaard Frederiksen <email@example.com>, Hayley G Evans, Klaus S Frederiksen, Leonie S Taams