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E-GEOD-51540 - Effects of TNF-alpha blocking in sorted Th17 cells from co-cultures of human CD4-positive and CD14-positive cells

Status
Released on 1 November 2013, last updated on 18 November 2013
Organism
Homo sapiens
Samples (18)
Array (1)
Protocols (5)
Description
Human CD4+ T cells and CD14+ monocytes from healthy donors were co-cultured with anti-CD3 for three days in the presence or absence of TNF-alpha mAb (Adalimumab). Classical Th17 cells (Th17) or those generated in the presence of the inhibitor (iTh17) were then sorted and analyzed by full transcriptome microarray analysis. A total of 18 samples consisting of paired samples from 9 healthy volunteers were compared. Sorted Th17 cells from co-cultures containing either Adalimumab (1 microgram/ml) or not were obtained from each of the donors, and compared by full transcriptome profiling.
Experiment type
transcription profiling by array 
Contacts
Klaus Stensgaard Frederiksen <geo@ncbi.nlm.nih.gov>, Hayley G Evans, Klaus S Frederiksen, Leonie S Taams
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-51540.idf.txt
Sample and data relationshipE-GEOD-51540.sdrf.txt
Raw data (1)E-GEOD-51540.raw.1.zip
Processed data (1)E-GEOD-51540.processed.1.zip
Array designA-GEOD-16268.adf.txt
Links