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E-GEOD-47444 - Off-target effects of VEGF-A regulation by shRNAs

Status
Released on 6 August 2015, last updated on 19 August 2015
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
The aim of this project was to investigate how genome-wide gene expression patterns change when the expression of VEGF-A is modulated using different lentivirally delivered shRNA molecules that are complementary to VEGF-A promoter region. To study these changes, 12 gene expression array experiments were conducted with mouse endothelial cell line (C166). The C166 cells were transduced (20% confluency, MOI 10) with lentiviral vectors that all express GFP marker gene and two of them express additional shRNA molecule that is complementary to different regions in the VEGF-A promoter and either up-regulate (VEGF-up) or downregulate (VEGF-down) VEGF-A expression. Fresh medium was changed 72h after transduction. Cells were collected at day 7.
Experiment type
transcription profiling by array 
Contacts
Mikko Petri Turunen <mikko.turunen@uef.fi>, Mikko P Turunen, Tiia Husso
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-47444.idf.txt
Sample and data relationshipE-GEOD-47444.sdrf.txt
Raw data (1)E-GEOD-47444.raw.1.zip
Processed data (1)E-GEOD-47444.processed.1.zip
Additional data (1)E-GEOD-47444.additional.1.zip
Array designA-GEOD-16225.adf.txt
Links