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E-GEOD-46958 - Transcription profiling by array of roots of hydroponically grown Arabidopsis treated with 0.125 mM gold against untreated control to study the uptake of gold

Status
Released on 1 August 2013, last updated on 30 April 2015
Organism
Arabidopsis thaliana
Samples (6)
Array (1)
Protocols (7)
Description
Gold is widely considered to be a biologically inert element; however, it can elicit a profound biological response in plants. Plants can be exposed to significant levels of this precious metal in the environment from naturally occurring sources, as the result of mining activities or more recently resulting from the escalating use of nanoparticles in industry. In this microarray study we have investigated the gene expression response of Arabidopsis thaliana (Arabidopsis) to gold. Although the uptake of metal cations by plant transporters is well characterised, little is known about the uptake of gold, which exists in soil predominantly in a zero-valent state (Au0). We used this study to monitor the expression of candidate genes involved in metal uptake and transport. These show the down-regulation of a discreet number of genes known to be involved in the transport of copper, cadmium, nickel and iron. The experiment comprised three replicate jars of hydropnically-grown Arabidopsis, each treated with 0.125 mM KAuCl4, and three replicate jars of hydropnically-grown Arabidopsis which were treated with water only.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-46958.idf.txt
Sample and data relationshipE-GEOD-46958.sdrf.txt
Raw data (1)E-GEOD-46958.raw.1.zip
Processed data (1)E-GEOD-46958.processed.1.zip
Array designA-AFFY-2.adf.txt
Links