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E-GEOD-46189 - The spatio-temporal program of DNA replication is driven by specific epigenetic signatures in human cells

Status
Released on 1 May 2014, last updated on 31 May 2014
Organism
Homo sapiens
Samples (5)
Protocols (3)
Description
We report the application of single-molecule-based sequencing technology for high-throughput profiling of DNA replication origins in K562 mammalian cells. We have optimized a specific method of peak detection adapted to the signal produced by the sequencing of short nascent strands (SNS) that are specific of replication initiation, with the aim to have both a good sensitivity and specificity. We demonstrated the existence of the spatiao-temporal program of DNA replication driven by a specific epigenetic signature. K562 human cells; five samples subjected to SNS-Seq.
Experiment type
other 
Contacts
Jean-Charles Cadoret <cadoret@ijm.univ-paris-diderot.fr>, A Arneodo, B Audit, F Picard, J Poulain, J-C Cadoret, L Duret, M-N Prioleau
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-46189.idf.txt
Sample and data relationshipE-GEOD-46189.sdrf.txt
Additional data (1)E-GEOD-46189.additional.1.zip
Links