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E-GEOD-44899 - miRNA expression profiling in hereditary breast tumors

Status
Released on 21 June 2014, last updated on 28 June 2014
Organism
Homo sapiens
Samples (80)
Array (1)
Protocols (5)
Description
The objective of the study was to characterize hereditary breast tumors based on their miRNA expression profiles. To this end, we performed global miRNA expression analysis of more than 800 human miRNA genes in a large series of 80 FFPE breast tissue samples. The series included 66 hereditary breast primary tumors from 13 BRCA1 mutation carriers, 10 BRCA2 mutation carriers and 43 non-BRCA12 tumors denominated hereafter as BRCAX tumors. In addition we have analyzed 10 sporadic breast carcinomas and 4 normal breast tissues obtained after breast reduction surgery from healthy donors with no family history of breast cancer. To avoid contamination with normal breast tissue, tumoral area on FFPE blocks was marked by a pathologist and macrodissected for subsequent total RNA extraction. Microarray expression profiling was performed using miRCURY LNATM microRNA Array v.11 (Exiqon A/S, Denmark), in a single color experiments in a pairwise comparison experimantal design.
Experiment type
transcription profiling by array 
Contacts
Miljana Tanic <tanic.miljana@gmail.com>, Ana Osorio, Beatriz Martinez-Delgado, Ivan Marquez-Rodas, Javier Benitez, Maria S Rodriguez-Pinilla, Victoria Fernandez
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-44899.idf.txt
Sample and data relationshipE-GEOD-44899.sdrf.txt
Raw data (1)E-GEOD-44899.raw.1.zip
Processed data (1)E-GEOD-44899.processed.1.zip
Array designA-MEXP-1519.adf.txt
Links