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E-GEOD-44724 - Genome-wide maps of CENP-A nucleosomes in three neocentromere-containing cell lines

Status
Released on 4 May 2013, last updated on 3 May 2014
Organism
Homo sapiens
Samples (6)
Protocols (3)
Description
We used native ChIP-seq of CENP-A-containing particles from normal centromeres on alpha-satellite DNA and three naturally-occurring neocentromeres to test the proposed models for the major form of the fundamental repeating unit of centromeric chromatin. We found that the predominant form of the CENP-A particle at the centromere is an octameric nucleosome with loose terminal DNA. Additionally, we found CENP-A nucleosomes are strongly phased on the 171 bp alpha-satellite monomers of normal centromeres, and also display strong positioning and neocentromeres. Comparison of CENP-A and bulk nucleosome DNA lengths and positions in three different human neocentromere-containing cell lines
Experiment type
ChIP-seq 
Contacts
Ben Black
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-44724.idf.txt
Sample and data relationshipE-GEOD-44724.sdrf.txt
Processed data (1)E-GEOD-44724.processed.1.zip
Links