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E-GEOD-42216 - Gene expression analysis of the immortalized human endothelial cell lines HMEC-1 and TIME

Status
Released on 11 September 2013, last updated on 26 September 2013
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (7)
Description
The distinction between lymphatic and blood vessels is biologically fundamental. Two immortalized cell lines, which have been widely used as models for endothelial cells of blood vascular origin, are the human microvascular endothelial cell line-1 (HMEC-1) and the telomerase-immortalized microvascular endothelial cell line (TIME). However, analysis of protein expression by flow cytometry revealed expression of lymphatic markers on these cell lines. Furthermore, functional in vitro leukocyte transmigration assays demonstrated deficiencies in several steps of the leukocyte extravasation cascade. Hence we performed this microarray analysis of the gene expression in HMEC-1 and TIME. We then compare the expression profiles to those of published blood- and lymphatic endothelial cells. Analysis of the gene expression profile of the immortalized human endothelial cell lines HMEC-1 and TIME to be compared to published primary blood- and lymphatic endothelial cells (see supplementary file linked below).
Experiment type
transcription profiling by array 
Contacts
Heikki Irjala, Johannes Keuschnigg, John-Patrick Mpindi, Kaisa Auvinen, Marko Salmi, Olli Kallioniemi, Sampsa Hautaniemi, Sirkku Karinen, Sirpa Jalkanen
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-42216.idf.txt
Sample and data relationshipE-GEOD-42216.sdrf.txt
Raw data (1)E-GEOD-42216.raw.1.zip
Processed data (1)E-GEOD-42216.processed.1.zip
Additional data (1)E-GEOD-42216.additional.1.zip
Array designA-GEOD-16273.adf.txt
Links