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E-GEOD-41443 - Expression data in H1299 after retinoblastoma binding protein-2 (RBP2) knockdown

Status
Released on 20 June 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
To identify genes whose expression was affected by RBP2, we performed gene expression microarray analysis in control and RBP2 depletion human lung cancer cell line. Two individual shRNA (RBP2 KD1 and RBP2 KD2) were used to limit potential off-target effects. One scramble short haripin RNA (shRNA) and two individual shRNAs to the human RBP2 gene (RBP2 KD1 and RBP2 KD2) were used. Two replicates per group were used. H1299 cells were infected with lentivirus which carriy shRNAs for 3 days. RNA was isloated by Trizol reagent and analyzed by bioanalyzer. Microarray experiments was performed by the Affymetrix Gene Expression Service Laboratory at Academia Sinica.
Experiment type
transcription profiling by array 
Contacts
Citation
Histone Demethylase RBP2 Promotes Lung Tumorigenesis and Cancer Metastasis. Teng YC, Lee CF, Li YS, Chen YR, Hsiao PW, Chan MY, Lin FM, Huang HD, Chen YT, Jeng YM, Hsu CH, Yan Q, Tsai MD, Juan LJ. , PMID:23722541
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-41443.idf.txt
Sample and data relationshipE-GEOD-41443.sdrf.txt
Raw data (1)E-GEOD-41443.raw.1.zip
Processed data (1)E-GEOD-41443.processed.1.zip
Array designA-AFFY-44.adf.txt
Links