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E-GEOD-39967 - Transcriptional profiles of unfractionated lymph nodes cells obtained from completely protected, non protected and unvaccinated control rhesus macaques seven days prior to, and four and fourteen days after wt SIVmac239 challenge by microarray analysis

Status
Released on 8 August 2012, last updated on 20 August 2012
Organism
Macaca mulatta
Samples (85)
Array (1)
Protocols (6)
Description
Live-attenuated SIV vaccines (LAVs) remain the most efficacious of all vaccines in nonhuman primate models of HIV/AIDS, yet the basis of their robust protection remains poorly understood. Here, we demonstrate that the degree of LAV-mediated protection against intravenous (IV) wildtype (wt) SIVmac239 challenge strongly correlates with the magnitude and function of SIV-specific, effector-differentiated T cells in lymph node (LN), but not with such T cell responses in blood or with other cellular, humoral and innate immune parameters. Maintenance of protective T cell responses was associated with persistent LAV replication in LN, which occurred almost exclusively in follicular helper T cells. Thus, effective LAVs maintain lymphoid tissue-based, effector-differentiated, SIV-specific T cells that intercept and suppress early wt SIV amplification and, if present in sufficient frequencies, can completely control and perhaps clear infection -- an observation that provides rationale for development of safe, persistent vectors that can elicit and maintain such responses. There are four protection outcome groups: unvaccinated control (C), complete protect (CP), non-protect (NP) and partial protect (PP). Respectively for these groups, there are 7, 9, 6 and 4 animals sampled seven days pre-challenge (minus7PCD); 9, 13, 5 and 5 animals sampled four days post-challenge (4PCD) and 8, 11, 5 and 3 fourteen days post-challenge (14PCD).
Experiment type
transcription profiling by array 
Contacts
Francois Lefebvre <flefebvre@vgtifl.org>, Adrian McDermott, Alfred W Legasse, Andrew Sylwester, Andrew T Smith, David C Montefiori, Eisa Mahyari, Guido Ferrari, Haesun Park, Jeffrey D Lifson, Jin Y Bae, Kelli Oswald, Louis J Picker, Marcelo Delos Reyes, Mark J Cameron, Michael K Axthelm, Michael Piatak, Noel Coombes, Paul T Edlefsen, Petra Stafova, Rafick P Sékaly, Rebecca Shoemaker, Richard Lum, Scott G Hansen, Shoko Hagen, Tonya Swanson, Yoshinori Fukazawa, Yuan Li
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-39967.idf.txt
Sample and data relationshipE-GEOD-39967.sdrf.txt
Processed data (1)E-GEOD-39967.processed.1.zip
Additional data (1)E-GEOD-39967.additional.1.zip
Array designA-MEXP-1172.adf.txt
Links