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E-GEOD-39899 - Gene expression profiling in B lymphocytes from transplanted patients

Status
Released on 1 August 2015, last updated on 19 August 2015
Organism
Homo sapiens
Samples (32)
Array (1)
Protocols (6)
Description
The achievement of a drug-free operational tolerance for renal transplanted patients is a major goal in organ transplantation. Previous gene expression profiling in peripheral blood mononuclear cells (PBMC) identified genes associated with operational tolerance. The identification of a common pattern of B cell-related genes associated with tolerance encourage us to analyze gene expression in purified B cell from operationally tolerant patients (TOL=10) compared to renal transplanted patients with stable graft function (STA=12) under immunosuppression and also compared to healthy volunteers (HV=10) who have no immunosuppressive treatment and no graft. Microarray analyses exhibited an absence of gene signature associated with tolerance in purified B cell compared to STA or HV. These results suggest that the B cell signatures observed in PBMC may be due to an increase number of total B cells rather than specific B cell characteristics in operationally tolerant patients. This dataset represents gene expression profiling of purified B cells from 10 renal transplanted patients with operational tolerance (TOL), 12 renal transplanted patients with stable graft function under immunosuppression (STA) and 10 healthy volunteers (HV).
Experiment type
transcription profiling by array 
Contacts
Richard Danger <richard.danger@univ-nantes.fr>, Mélanie Chesneau, Sophie Brouard
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-39899.idf.txt
Sample and data relationshipE-GEOD-39899.sdrf.txt
Processed data (1)E-GEOD-39899.processed.1.zip
Additional data (2)E-GEOD-39899.additional.1.zip, E-GEOD-39899.additional.2.zip
Array designA-GEOD-13607.adf.txt
Links