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E-GEOD-36057 - Lung Natural Helper cells

Status
Released on 24 February 2012, last updated on 6 March 2012
Organism
Mus musculus
Samples (5)
Array (1)
Protocols (7)
Description
Natural Helper cells constitute a unique lineage of Th2-cytokine producting innate lymphocytes, here we characterize the gene expression profile of non-stimulated or PMA/ionomycin-stimulated Natural Helper cells from naive C57Bl/6 mouse lungs. We used microarrays to detail the gene expression profile of stimulated and unstimulated lung Natural Helper cells in mice. Lineage(-)Sca-1(+)c-Kit(-/low)CD127(+)CD25(+) Lung Natural Helper cells were purified from naive 6-8 week old B6 mice by FACS. RNA was extracted immediately from un-stimulated Natural Helper cells after FACS. Stimulated Natural Helper cells were cultured with PMA/ionomycin for 3 days followed by RNA extraction.
Experiment type
transcription profiling by array 
Contacts
Timotheus You Fu Halim <tim.halim@hotmail.com>, Fumio Takei, Timotheus Y Halim
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-36057.idf.txt
Sample and data relationshipE-GEOD-36057.sdrf.txt
Raw data (1)E-GEOD-36057.raw.1.zip
Processed data (1)E-GEOD-36057.processed.1.zip
Array designA-AFFY-130.adf.txt
Links