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E-GEOD-35477 - CD98 marks a subpopulation of head and neck squamous cell carcinoma cells with stem cell properties

Status
Released on 24 January 2015, last updated on 3 February 2015
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (7)
Description
The application of compounds specifically targeting the cancer stem cells (CSCs), in addition to routinely used therapeutics, would likely improve the clinical outcome of head and neck squamous cell carcinoma (HNSCC). The previously described monoclonal antibody K984 (now found to recognize CD98) was used to identify, select and isolate a specific tumor subpopulation. These cells were molecularly characterized and subjected to serial transplantation experiments to determine the CSC properties. We show that CD98high cells, in contrast to CD98low cells, are able to generate tumors in immunodeficient mice and that the CD98high subpopulation expresses high levels of cell cycle control and DNA repair genes, while the CD98low fraction shows expression patterns that represent the more differentiated cells forming the bulk of the tumor. Transcriptional profiling of CD98high cells (n=4 fractions) of VU-SCC-OE xenograft tumors compared to CD98low cells (n=3 fractions) from the corresponding tumors.
Experiment type
transcription profiling by array 
Contacts
Daoud Sie <d.sie@vumc.nl>, Arjen Brink, Boudewijn J Braakhuis, François Rustenburg, Gerrit J Schuurhuis, J M Damen, Marijke Stigter-van Walsum, Monique Slijper, Ruud H Brakenhoff, Sanne R Martens-de Kemp, Thijs Wu, Wessel N van Wieringen
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-35477.idf.txt
Sample and data relationshipE-GEOD-35477.sdrf.txt
Raw data (1)E-GEOD-35477.raw.1.zip
Processed data (1)E-GEOD-35477.processed.1.zip
Array designA-AGIL-28.adf.txt
Links