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E-GEOD-35340 - Notch is active in Langerhans Cell Histiocytosis and confers pathognomonic features on dendritic cells

Status
Released on 24 October 2012, last updated on 29 October 2012
Organism
Homo sapiens
Samples (17)
Array (1)
Protocols (6)
Description
Langerhans cell histiocytosis (LCH) is a disease characterized by the accumulation of eponymous CD1a+ Langerin+ Langerhans-cell (LC)-like dendritic cells (DC) of largely unknown origin. Here we have performed comparative transcriptome analysis of highly purified CD207+/CD1a+ Langerhans cell histiocytosis (LCH) cells derived from different locations and disease courses and three major human dendritic cell lineages: epidermal Langerhans cells, myeloid dendritic cells (mDC1) and plasmacytoid dendritic cells (pDC) in order to investigate the relationship between LCH cells and naturally occurring dendritic cells. Data obtained indicate that LCH cells form a distinct DC entity. Furthermore, we have identified transcripts that are uniquely expressed by LCH cells in comparison to LC, mDC1, and pDC, and induce LCH-specific features in human DC. Primary cells were isolated from peripheral blood (mDC1 and pDC), skin (epidermal Langerhans cells) and CD207+/CD1a+ Langerhans cell histiocytosis (LCH) cells derived from different locations. RNA was isolated from these cells ex vivo.
Experiment type
transcription profiling by array 
Contacts
Maximilian Kauer <maximilian.kauer@ccri.at>, Caroline Hutter, Ernst Kriehuber
Citation
Notch is active in Langerhans cell histiocytosis and confers pathognomonic features on dendritic cells. Hutter C, Kauer M, Simonitsch-Klupp I, Jug G, Schwentner R, Leitner J, Bock P, Steinberger P, Bauer W, Carlesso N, Minkov M, Gadner H, Stingl G, Kovar H, Kriehuber E. , PMID:23074278
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-35340.idf.txt
Sample and data relationshipE-GEOD-35340.sdrf.txt
Raw data (1)E-GEOD-35340.raw.1.zip
Processed data (1)E-GEOD-35340.processed.1.zip
Array designA-AFFY-44.adf.txt
Links